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A BAC/BIBAC-based, Integrated Physical

and Genetic Map of Soybean

We have developed a genome-wide, BAC/BIBAC-based, integrated genetic and physical map of the soybean genome from five BAC and BIBAC libraries (BAC & EST Unigene Resources) of Glycine max cvs. Forrest, Williams 82 and Faribault. This study was supported in part by the National Science Foundation – Plant Genome Program (Award #: 9872635), the Illinois Soybean Operating Board (Award #: ISPOB-24-198-3) and Texas Agricultural Experiment Station (Acc. #: 8536-203104).

The BIBACs were cloned in a plant-transformation binary vector (pCLD04541), and are suited for plant transformation via Agrobacterium; and the BACs cloned in pECBAC1 and pBeloBAC 11 are suited for large-scale genome sequencing because of their small vector size (7.5 kb).

 

Map Summary

Contigs in # of clones

Date

Clone

Cov

(1 x)

Marker

Total

Mb

Single

Contig

>100

100:51

50:26

25:10

9:3

2

12/01

75568

8.7

0

1667

5884

5488

18

68

134

3038

1845

385

03/02

78001

9.6

278

1600

5918

5253

20

109

106

3226

1687

105

08/02

78001

9.6

388

1408

4954

2905

94

321

504

920

850

216

To access all BAC fingerprints and conduct e-chromosome walking, or build overlapping clone path tiles by yourself, starting from a particular clone, click:
                            Soybean Clone -> FPC Hitting Tool
To search the contig containing a particular clone, click:
                            Soybean Clone -> Contig
To access all contigs based on Contig No., click:
                           Soybean Contig No. -> Contig Map

To access the contigs anchored to the genetic map from a particular DNA marker, click:
                            Soybean Integrated Physical and Genetic Map
To access all positive clones of a DNA marker or EST and the contig(s) containing them, click:

                            Soybean Marker/EST -> Positive Clones
                                -> Contig/Fingerprint FPC Hit Matches

To determine the overlapping relationships of a group of selected clones of interest, click:
                            Soybean Clone -> Fingerprint Map
 

 
Last Updated Thursday December 19, 2002 Questions ? webmaster@hbz.tamu.edu  
 
Copyright © 2002 hbz laboratory all rights reserved.
Norman E. Borlaug Center, College Station, TX77843